Optimization of Moringa Oleifera Leaf Extraction and Investigation of Anti Breast Cancer Activity with the Leaf Extract

Authors

  • Nazia Hossain IIUM
  • M. E. S. Mirghani IIUM
  • Raha Bt. Raus IIUM

DOI:

https://doi.org/10.18034/ei.v3i2.194

Keywords:

Moringa oleifera leaves extract, Breast Cancer Inhibition, Anti-cancer Activity, Optimization, Response Surface Methodology

Abstract

Moringa oleifera (M. oleifera) has been considered as medicinal plant and food substance since ancient times. Nowadays various therapeutic effects of M. oleifera such as antimicrobial, anticancer, anti-inflammatory, antidiabetic, and antioxidant effects have been successfully investigated. In this study, the main objective was to optimize M. oleifera leaf-methanol extraction using Design of Experiment (DoE) software with response surface methodology in terms of temperature and incubation time by sonication. The other objective was to investigate anti-cancer activity (breast cancer cell: MCF-7) by M. oleifera methanol extraction incorporation with microtitrate tetrazolium (MTT) assay. In this research, the optimum condition for leaf extraction was found to be temperature 50⁰C and incubation time 45 min with medium frequency at sonication and it obtained 88.39% cancer cell growth inhibition by this condition. Extracted leaves inhibited MCF-7 cell line with 87.13% in average at wavelength A570nm.

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Author Biographies

Nazia Hossain, IIUM

Department of Biotechnology-Biochemical Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM), Gombak, MALAYSIA

and

International Institute for Halal Research and Training (INHART), IIUM, Gombak, MALAYSIA

M. E. S. Mirghani, IIUM

Department of Biotechnology-Biochemical Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM), Gombak, MALAYSIA

and

International Institute for Halal Research and Training (INHART), IIUM, Gombak, MALAYSIA

Raha Bt. Raus, IIUM

Department of Biotechnology-Biochemical Engineering, Faculty of Engineering, International Islamic University Malaysia (IIUM), Gombak, MALAYSIA

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Published

2015-12-30

How to Cite

Hossain, N., Mirghani, M. E. S., & Raus, R. B. (2015). Optimization of Moringa Oleifera Leaf Extraction and Investigation of Anti Breast Cancer Activity with the Leaf Extract. Engineering International, 3(2), 97–104. https://doi.org/10.18034/ei.v3i2.194

Issue

Vol. 3 No. 2 (2015): July - December

Section

Peer Reviewed Articles

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